A Polycomb repressive complex is required for RNAi-mediated heterochromatin formation and dynamic distribution of nuclear bodies

Author:

Xu Jing1,Zhao Xiaolu23,Mao Fengbiao2,Basrur Venkatesha4,Ueberheide Beatrix5,Chait Brian T5,Allis C David6,Taverna Sean D7,Gao Shan3,Wang Wei1,Liu Yifan2ORCID

Affiliation:

1. School of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China

2. Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA

3. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China

4. Proteomics Resource Facility, Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA

5. Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, the Rockefeller University, New York, NY 10065, USA

6. Laboratory of Chromatin Biology and Epigenetics, the Rockefeller University, New York, NY 10065, USA

7. Department of Pharmacology and Molecular Sciences and the Center for Epigenetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Abstract

Abstract Polycomb group (PcG) proteins are widely utilized for transcriptional repression in eukaryotes. Here, we characterize, in the protist Tetrahymena thermophila, the EZL1 (E(z)-like 1) complex, with components conserved in metazoan Polycomb Repressive Complexes 1 and 2 (PRC1 and PRC2). The EZL1 complex is required for histone H3 K27 and K9 methylation, heterochromatin formation, transposable element control, and programmed genome rearrangement. The EZL1 complex interacts with EMA1, a helicase required for RNA interference (RNAi). This interaction is implicated in co-transcriptional recruitment of the EZL1 complex. Binding of H3K27 and H3K9 methylation by PDD1—another PcG protein interacting with the EZL1 complex—reinforces its chromatin association. The EZL1 complex is an integral part of Polycomb bodies, which exhibit dynamic distribution in Tetrahymena development: Their dispersion is driven by chromatin association, while their coalescence by PDD1, likely via phase separation. Our results provide a molecular mechanism connecting RNAi and Polycomb repression, which coordinately regulate nuclear bodies and reorganize the genome.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shanxi Province

University of Michigan

NIH

Rockefeller University

NSF

Johns Hopkins University School of Medicine

Marine S&T Fund of Shandong Province

Fundamental Research Funds for the Central Universities

University of Southern California Keck School of Medicine

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference132 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3