Affiliation:
1. Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu, China
2. State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, Gansu, China
Abstract
Abstract
MicroRNAs (miRNAs) have been reported to play indispensable roles in regulating various developmental processes via the posttranscriptional repression of target genes in insect species. In the present paper, we studied the miRNAs in Indian meal moth (Plodia interpunctella (Hübener)), one of the most economically important stored grains pests around the world. In total, 12 small RNA libraries from four developmental stages of P. interpunctella were constructed, and 178 known and 23 novel miRNAs were identified. In addition, the expression profiles of these miRNAs were assessed across different developmental stages and miRNAs that were highly expressed in eggs, larvae, pupae, and adults were identified. Specifically, 100, 61, and 52 miRNAs were differentially expressed between eggs and larvae, larvae and pupae, and pupae and adults, respectively. The KEGG and GO analysis of the predicted target genes suggested the essential roles of miRNAs in the regulation of complex development of P. interpunctella. Importantly, we also found a set of miRNAs might be involved in the larval metamorphic molting process, with their expressions increasing and then decreasing during the larva-pupa-adult stages of P. interpunctella. In conclusion, the current paper has discovered numerous miRNAs, and some key miRNAs that might be responsible for regulating development in P. interpunctella. To our knowledge, this is the first study to document miRNAs and their expression patterns in interpunctella, and those findings would lay an important molecular foundation for future functional analysis of these miRNAs in P. interpunctella.
Funder
National key Research and Development Program of China
Natural Science Foundation of the Jiangsu Higher Education Institutions of China
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Insect Science,Ecology,General Medicine
Reference50 articles.
1. MicroRNA functions in insects;Asgari;Insect Biochem. Mol. Biol,2013
2. Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs;Bartel;Nat. Rev. Genet,2004
3. Correlated expression patterns of microRNA genes with age-dependent behavioural changes in honeybee;Behura;Insect Mol. Biol,2010
4. miR-9a prevents apoptosis during wing development by repressing Drosophila LIM-only;Bejarano;Dev. Biol,2010
5. Micrornas and the evolution of insect metamorphosis;Belles;Annu. Rev. Entomol,2017
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献