Mild hydrostatic-pressure (15 MPa) affects the assembly, but not the growth, of oil-degrading coastal microbial communities tested under limiting conditions (5°C, no added nutrients)

Abstract

ABSTRACT Hydrostatic pressures (HP) <30–40 MPa are often considered mild, and their impact on petroleum biodegradation seldom considered. However, the frequent use of nutrient-rich media in lab-scale high-pressure reactors may exaggerate HP importance by resulting in a strong growth stimulation as compared to oligotrophic marine environments. Here, we tested coastal seawater microbial communities, presumably enriched in pressure-sensitive microorganisms. Limiting environmental conditions for growth were applied (i.e. low temperature [5°C], no added nutrients) and HP tested at 0.1 and 15 MPa, using crude oils from three different reservoirs. The cell number was not affected by HP contrary to the microbial community composition (based on 16S rRNA gene and 16S rRNA sequences). The most predominant genera were Zhongshania, Pseudomonas and Colwellia. The enrichment of Zhongshania was crude-oil dependent and comparable at 0.1 and 15 MPa, thus showing a piezotolerant phenotype under the present conditions; Pseudomonas’ was crude-oil dependent at 0.1 MPa but unclear at 15 MPa. Colwellia was selectively enriched in the absence of crude oil and suppressed at 15 MPa. HP shaped the assemblage of oil-degrading communities even at mild levels (i.e. 15 MPa), and should thus be considered as a fundamental factor to assess oil bioremediation along the water column.