Nested association mapping reveals the genetic architecture of spike emergence and anthesis timing in intermediate wheatgrass

Author:

Altendorf Kayla R1ORCID,Larson Steven R2,DeHaan Lee R3,Crain Jared4ORCID,Neyhart Jeff5ORCID,Dorn Kevin M6,Anderson James A7

Affiliation:

1. USDA-ARS, Forage Seed and Cereal Research Unit, Irrigated Agriculture Research and Extension Center, Prosser, WA 99350, USA

2. The Land Institute, Salina, KS 67401, USA

3. USDA-ARS, Forage Range and Research Lab, Utah State University, Logan, UT 84322, USA

4. Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA

5. GEMS Informatics Initiative, University of Minnesota, St. Paul, MN 55108, USA

6. USDA-ARS, Soil Management and Sugarbeet Research, Fort Collins, CO 80526, USA

7. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA

Abstract

Abstract Intermediate wheatgrass (Thinopyrum intermedium) is an outcrossing, cool season grass species currently undergoing direct domestication as a perennial grain crop. Though many traits are selection targets, understanding the genetic architecture of those important for local adaptation may accelerate the domestication process. Nested association mapping (NAM) has proven useful in dissecting the genetic control of agronomic traits many crop species, but its utility in primarily outcrossing, perennial species has yet to be demonstrated. Here, we introduce an intermediate wheatgrass NAM population developed by crossing ten phenotypically divergent donor parents to an adapted common parent in a reciprocal manner, yielding 1,168 F1 progeny from 10 families. Using genotyping by sequencing, we identified 8,003 SNP markers and developed a population-specific consensus genetic map with 3,144 markers across 21 linkage groups. Using both genomewide association mapping and linkage mapping combined across and within families, we characterized the genetic control of flowering time. In the analysis of two measures of maturity across four separate environments, we detected as many as 75 significant QTL, many of which correspond to the same regions in both analysis methods across 11 chromosomes. The results demonstrate a complex genetic control that is variable across years, locations, traits, and within families. The methods were effective at detecting previously identified QTL, as well as new QTL that align closely to the well-characterized flowering time orthologs from barley, including Ppd-H1 and Constans. Our results demonstrate the utility of the NAM population for understanding the genetic control of flowering time and its potential for application to other traits of interest.

Funder

Perennial Agriculture Project in conjunction with the Malone Family Land Preservation Foundation and The Land Institute

Publisher

Oxford University Press (OUP)

Subject

Genetics(clinical),Genetics,Molecular Biology

Reference80 articles.

1. Floret site utilization and reproductive tiller number are primary components of grain yield in intermediate wheatgrass spaced plants

2. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs;Altschul;Nucleic Acids Res,1997

3. Nested association mapping of stem rust resistance in wheat using genotyping by sequencing;Bajgain;PLoS ONE,2016

4. Genome-wide association study of yield component traits in intermediate wheatgrass and implications in genomic selection and breeding;Bajgain;G3 Genes, Genomes, Genetics,2019

5. Dominance and G×E interaction effects improve genomic prediction and genetic gain in intermediate wheatgrass (Thinopyrum intermedium);Bajgain;Plant Genome. 1-,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3