Single-nucleus RNA sequencing and mRNA hybridization indicate key bud events and LcFT1 and LcTFL1-2 mRNA transportability during floral transition in litchi

Author:

Yang Ming-Chao1ORCID,Wu Zi-Chen2ORCID,Chen Ri-Yao3,Abbas Farhat1ORCID,Hu Gui-Bing1ORCID,Huang Xu-Ming1ORCID,Guan Wei-Song1,Xu Yi-Song2,Wang Hui-Cong14ORCID

Affiliation:

1. Guangdong Laboratory for Lingnan Modern Agriculture/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, College of Horticulture, South China Agricultural University , Guangzhou , China

2. Becton Dickinson Medical Devices (Shanghai) Co., Ltd , Guangzhou, Guangdong, 510180 , China

3. College of Engineering, South China Agricultural University , Guangzhou , China

4. Department of Life Sciences and Technology, Yangtze Normal University , Fuling 408100 , China

Abstract

Abstract In flowering plants, floral induction signals intersect at the shoot apex to modulate meristem determinacy and growth form. Here, we report a single-nucleus RNA sequence analysis of litchi apical buds at different developmental stages. A total of 41 641 nuclei expressing 21 402 genes were analyzed, revealing 35 cell clusters corresponding to 12 broad populations. We identify genes associated with floral transition and propose a model that profiles the key events associated with litchi floral meristem identity by analyzing 567 identified floral meristem cells at single cell resolution. Interestingly, single-nucleus RNA-sequencing data indicated that all putative FT and TFL1 genes were not expressed in bud nuclei, but significant expression was detected in bud samples by RT–PCR. Based on the expression patterns and gene silencing results, we highlight the critical role of LcTFL1-2 in inhibiting flowering and propose that the LcFT1/LcTFL1-2 expression ratio may determine the success of floral transition. In addition, the transport of LcFT1 and LcTFL1-2 mRNA from the leaf to the shoot apical meristem is proposed based on in situ and dot-blot hybridization results. These findings allow a more comprehensive understanding of the molecular events during the litchi floral transition, as well as the identification of new regulators.

Funder

Laboratory of Lingnan Modern Agriculture Project

Guangzhou Science and Technology Project

China Litchi and Longan Industry Technology Research System

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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