A pear S1-bZIP transcription factor PpbZIP44 modulates carbohydrate metabolism, amino acid, and flavonoid accumulation in fruits

Author:

Wang Hong12ORCID,Xu Kexin12,Li Xiaogang2,Blanco-Ulate Bárbara3,Yang Qingsong2,Yao Gaifang4,Wei Yiduo3,Wu Jun1ORCID,Sheng Baolong2,Chang Youhong2,Jiang Cai-Zhong35ORCID,Lin Jing12

Affiliation:

1. Nanjing Agricultural University College of Horticulture, , Nanjing 210014, China

2. Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Institute of Pomology, , Nanjing 210014, China

3. University of California, Davis Department of Plant Sciences, , Davis, CA 95616, USA

4. Hefei University of Technology School of Food and Biological Engineering, , Hefei 230009, China

5. United States Department of Agriculture, Agricultural Research Service Crops Pathology and Genetics Research Unit, , Davis, California, 95616, USA

Abstract

Abstract Fruit quality is defined by attributes that give value to a commodity. Flavor, texture, nutrition, and shelf life are key quality traits that ensure market value and consumer acceptance. In pear fruit, soluble sugars, organic acids, amino acids, and total flavonoids contribute to flavor and overall quality. Transcription factors (TFs) regulate the accumulation of these metabolites during development or in response to the environment. Here, we report a novel TF, PpbZIP44, as a positive regulator of primary and secondary metabolism in pear fruit. Analysis of the transient overexpression or RNAi-transformed pear fruits and stable transgenic tomato fruits under the control of the fruit-specific E8 promoter demonstrated that PpZIP44 substantially affected the contents of soluble sugar, organic acids, amino acids, and flavonoids. In E8::PpbZIP44 tomato fruit, genes involved in carbohydrate metabolism, amino acid, and flavonoids biosynthesis were significantly induced. Furthermore, in PpbZIP44 overexpression or antisense pear fruits, the expression of genes in the related pathways was significantly impacted. PpbZIP44 directly interacted with the promoter of PpSDH9 and PpProDH1 to induce their expression, thereby depleting sorbitol and proline, decreasing citrate and malate, and enhancing fructose contents. PpbZIP44 also directly bound to the PpADT and PpF3H promoters, which led to the carbon flux toward phenylalanine metabolites and enhanced phenylalanine and flavonoid contents. These findings demonstrate that PpbZIP44 mediates multimetabolism reprogramming by regulating the gene expression related to fruit quality compounds.

Funder

Jiangsu Province Seed Industry Revitalization Unveiled Project: Breeding of New Pear Cultivars with High-quality and Extreme Early Ripening

Jiangsu Agricultural Key New Varieties Innovation

Jiangsu Modern Agricultural Industry Technology System

General Program of National Natural Science Foundation of China

Jiangsu Agricultural Science and Technology Independent Innovation Fund

Publisher

Oxford University Press (OUP)

Subject

Horticulture,Plant Science,Genetics,Biochemistry,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3