Discovery of novel Mamastroviruses in Bactrian camels and dromedaries reveals complex recombination history

Author:

Qureshi Muhammad I1,Worthington Brian M123ORCID,Liu Yongmei123,Cheung William Y.-M123ORCID,Su Shuo4,Zheng Zuoyi1,Li Lifeng123,Lam Tommy T -Y1235,Guan Yi1235,Zhu Huachen1235ORCID

Affiliation:

1. Guangdong-Hong Kong Joint Laboratory of Emerging Infectious Diseases/MOE Joint Laboratory for International Collaboration in Virology and Emerging Infectious Diseases, Joint Institute of Virology (Shantou University/The University of Hong Kong), Shantou University , 243 Daxue Road, Shantou, Guangdong 515063, China

2. State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong , 5/F, Lab Block, 21 Sassoon Road, Pokfulam, Hong Kong SAR 000, China

3. Exploration, Knowledge, Intelligence and Health, Gewuzhikang (EKIH) Pathogen Research Institute , 13/F, Building 3, 3 Binglang Road, Futian District, Shenzhen, Guangdong 518045, China

4. Ministry of Education (MOE), Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunity, Institute of Immunology, College of Veterinary Medicine, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University , 1 Weigang Road, Nanjing, Jiangsu 210095, China

5. Laboratory of Data Discovery for Health Limited , 12/F, Building 19W, 19 Science Park West Avenue, Hong Kong Science Park, Pak Shek Kok, New Territories, Hong Kong SAR 000, China

Abstract

Abstract Virus emergence may occur through interspecies transmission and recombination of viruses coinfecting a host, with potential to pair novel and adaptive gene combinations. Camels are known to harbor diverse ribonucleic acid viruses with zoonotic and epizootic potential. Among them, astroviruses are of particular interest due to their cross-species transmission potential and endemicity in diverse host species, including humans. We conducted a molecular epidemiological survey of astroviruses in dromedaries from Saudi Arabia and Bactrian camels from Inner Mongolia, China. Herein, we deployed a hybrid sequencing approach coupling deep sequencing with rapid amplification of complementary deoxyribonucleic acid ends to characterize two novel Bactrian and eight dromedary camel astroviruses, including both partial and complete genomes. Our reported sequences expand the known diversity of dromedary camel astroviruses, highlighting potential recombination events among the astroviruses of camelids and other host species. In Bactrian camels, we detected partially conserved gene regions bearing resemblance to human astrovirus types 1, 4, and 8 although we were unable to recover complete reading frames from these samples. Continued surveillance of astroviruses in camelids, particularly Bactrian species and associated livestock, is highly recommended to identify patterns of cross-species transmission and to determine any epizootic threats and zoonotic risks posed to humans. Phylogenomic approaches are needed to investigate complex patterns of recombination among the astroviruses and to infer their evolutionary history across diverse host species.

Funder

Government of Guangdong Province

National Key Plan for Scientific Research and Development of China

Li Ka Shing Foundation

Shenzhen Government

National Center for International Research of Genomics

National Natural Science Foundation of China

Publisher

Oxford University Press (OUP)

Subject

Virology,Microbiology

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