Comparable and adaptable strategies to waterlogging stress regulated by adventitious roots between two contrasting species

Abstract

Abstract Cleistocalyx operculatus and Syzygium cumini possess a certain waterlogging tolerance. However, the comparable and adaptable strategies to waterlogging stress between these two species on the basis of waterlogging adventitious root (AR) regulation were still unclear. In this study, the plant performance in response to AR regulation based on AR removal (AR-R) and exogenous hormone application was investigated in terms of plant morphology, physiology, photosynthesis and AR traits. Results showed that C. operculatus possesses stronger waterlogging tolerance than S. cumini based on waterlogging tolerance coefficient, which is mainly due to the higher root biomass, root porosity and length, and activity of ARs, and shorter emergence time of ARs in C. operculatus than in S. cumini. The AR-R treatment increased activity and porosity of primary root, and induced a large amount of up-vertical ARs from the primary root systems in C. operculatus, while similar adaptive morphological changes in roots did not occur in AR-R-treated S. cumini. Exogenous abscisic acid (ABA) application had better effects on alleviating waterlogging damages than exogenous auxin (IAA) in balancing endogenous hormones (ABA and zeatin riboside), promoting AR development (porosity and activity, and the ratio of cortex area to stele area), improving the photosynthesis process and the antioxidant system (soluble protein, free proline and peroxidase). Moreover, under waterlogging conditions, exogenous ABA application induced greater increases in net photosynthesis rate, stomatal conductance, chlorophyll b and carotenoid in S. cumini than in C. operculatus, which suggested that S. cumini responded more positively and efficiently to exogenous ABA application than C. operculatus under waterlogging conditions. Thus, the findings provided new insights into the waterlogging adaptable strategies in waterlogging tolerant woody species on the basis of ARs and could provide scientific guidance for the application of these two species during revegetation activities in wetlands. Cleistocalyx operculatus could alternatively form a majority of up-vertical adventitious roots (ARs) from the primary roots after removing the normal ARs, but Syzygium cumini could not.Cleistocalyx operculatus possessed positive strategies to waterlogging stress, while S. cumini used traditional passive strategies.Exogenous abscisic acid (ABA) application had better effects on alleviating waterlogging damages in both species than exogenous auxin application.Syzygium cumini could more positively and efficiently respond to exogenous ABA application than C. operculatus.Waterlogging tolerance coefficient was significantly controlled by the chlorophyll contents and AR factors in C. operculatus and the AR factors and O2− in S. cumini.The best development of the AR number (ARN) and AR length (ARL) in exogenous ABA-treated C. operculatus may be closely related with positive zeatin riboside accumulation.The development of ARN and ARL was more important to waterlogging tolerance than that of AR porosity under waterlogging conditions.