Development, Validation, and Standardization of Polymerase Chain Reaction-Based Detection of E. coli O157-Reference-Cited by-同舟云学术

Development, Validation, and Standardization of Polymerase Chain Reaction-Based Detection of E. coli O157

Published:2004-05-01 Issue:3 Volume:87 Page:596-603
ISSN:1060-3271
Container-title:Journal of AOAC INTERNATIONAL
language:en
Short-container-title:

Author:

Abdulmawjood Amir¹,Bülte Michael¹,Roth Stefanie¹,Schönenbrücher Hahn¹,Cook Nigel²,Heuvelink Annet E³,Hoorfar Jeffrey⁴

Affiliation:

1. Justus-Liebig-University, Institute of Veterinary Food Sciences, Frankfurter str 92, 35392 Giessen, Germany

2. DEFRA Central Science Laboratory, Sand Hutton, York YO41 1LZ, United Kingdom

3. IHP, PO Box 9012, 7200 GN22, De Stoven, 7206 AX, Zutphen, The Netherlands

4. Danish Veterinary Institute, 27 Bulowsvej, DK-1790, Copenhagen 1790, Denmark

Abstract

Abstract A diagnostic polymerase chain reaction assay was developed for the detection of E. coli O157 as the first part of a multicenter validation and standardization project. The assay is based on amplification of sequences of the rfbE O157 gene and includes an internal amplification control. The selectivity of the assay was evaluated against 155 strains, including 32 E. coli O157, 38 E. coli non-O157, and 85 non-E. coli. It was shown to be highly inclusive (100%) and exclusive (100%). The assay has a 100% detection probability of approximately 2 × 103 cells per reaction.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Link

http://academic.oup.com/jaoac/article-pdf/87/3/596/32417814/jaoac0596.pdf

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