A comparison of DNA extraction methods used in the direct molecular detection of <i>Burkholderia pseudomallei</i> from blood-Reference-Cited by-同舟云学术

A comparison of DNA extraction methods used in the direct molecular detection of Burkholderia pseudomallei from blood

Published:2024-04-08 Issue:9 Volume:118 Page:629-631
ISSN:0035-9203
Container-title:Transactions of The Royal Society of Tropical Medicine and Hygiene
language:en
Short-container-title:

Author:

Mataira Georgia¹,Francis Fleur¹,Frazer Jaimie¹,Norton Robert¹²^ORCID

Affiliation:

1. Pathology Queensland, Townsville University Hospital , Douglas, Townsville, Queensland 4814 , Australia

2. Faculty of Medicine, University of Queensland , Brisbane , Australia

Abstract

Abstract Background Melioidosis is caused by Burkholderia pseudomallei. Direct molecular detection from unamplified blood remains insensitive. Methods Three different extraction methods—QIAamp UCP Pathogen Mini Kit, High Pure PCR template and MagNA Pure Pathogen Universal—were trialled using spiked human ethylenediaminetetraacetic acid blood. A type III secretion system 1 (TTSS-1) polymerase chain reaction was used for detection. Results The QIAamp UCP Pathogen Mini Kit performed best, with a limit of detection of 1.5×102 cfu/ml. Conclusions It is planned to use the QIAamp UCP Pathogen Mini Kit to do a larger study on blood collected from patients with melioidosis.

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/trstmh/article-pdf/118/9/629/58995263/trae020.pdf

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