Temperature response of denitrification and anammox reveals the adaptation of microbial communities to in situ temperatures in permeable marine sediments that span 50° in latitude
-
Published:2014-01-23
Issue:2
Volume:11
Page:309-320
-
ISSN:1726-4189
-
Container-title:Biogeosciences
-
language:en
-
Short-container-title:Biogeosciences
Author:
Canion A., Kostka J. E., Gihring T. M., Huettel M.ORCID, van Beusekom J. E. E., Gao H., Lavik G., Kuypers M. M. M.
Abstract
Abstract. Despite decades of research on the physiology and biochemistry of nitrate/nitrite-respiring microorganisms, little is known regarding their metabolic response to temperature, especially under in situ conditions. The temperature regulation of microbial communities that mediate anammox and denitrification was investigated in near shore permeable sediments at polar, temperate, and subtropical sites with annual mean temperatures ranging from −5 to 23 °C. Total N2 production rates were determined using the isotope pairing technique in intact core incubations under diffusive and simulated advection conditions and ranged from 2 to 359 μmol N m−2 d−1. For the majority of sites studied, N2 removal was 2–7 times more rapid under simulated advective flow conditions. Anammox comprised 6–14% of total N2 production at temperate and polar sites and was not detected at the subtropical site. Potential rates of denitrification and anammox were determined in anaerobic slurries in a temperature gradient block incubator across a temperature range of −1 °C to 42 °C. The highest optimum temperature (Topt) for denitrification was 36 °C and was observed in subtropical sediments, while the lowest Topt of 21 °C was observed at the polar site. Seasonal variation in the Topt was observed at the temperate site with values of 26 and 34 °C in winter and summer, respectively. The Topt values for anammox were 9 and 26 °C at the polar and temperate sites, respectively. The results demonstrate adaptation of denitrifying communities to in situ temperatures in permeable marine sediments across a wide range of temperatures, whereas marine anammox bacteria may be predominately psychrophilic to psychrotolerant. The adaptation of microbial communities to in situ temperatures suggests that the relationship between temperature and rates of N removal is highly dependent on community structure.
Publisher
Copernicus GmbH
Subject
Earth-Surface Processes,Ecology, Evolution, Behavior and Systematics
Reference57 articles.
1. Amano, T., Yoshinaga, I., Yamagishi, T., Thuoc, C. V., Thu, P. T., Ueda, S., Kato, K., Sako, Y., and Suwa, Y.: Contribution of Anammox Bacteria to Benthic Nitrogen Cycling in a Mangrove Forest and Shrimp Ponds, Haiphong, Vietnam, Microbes Environ., 26, 1–6, 2011. 2. An, S. M., Gardner, W. S., and Kana, T.: Simultaneous measurement of denitrification and nitrogen fixation using isotope pairing with membrane inlet mass spectrometry analysis, Appl. Environ. Microbiol., 67, 1171–1178, 2001. 3. Arnosti, C., Jorgensen, B. B., Sagemann, J., and Thamdrup, B.: Temperature dependence of microbial degradation of organic matter in marine sediments: polysaccharide hydrolysis, oxygen consumption, and sulfate reduction, Mar. Ecol.-Prog. Ser., 165, 59–70, 1998. 4. Bower, C. E. and Holm-Hansen, T.: A Salicylate-Hypochlorite Method for Determining Ammonia in Seawater, Can. J. Fish. Aquat. Sci., 37, 794–798, 1980. 5. Braman, R. S. and Hendrix, S. A.: Nanogram Nitrite and Nitrate Determination in Environmental and Biological-Materials by Vanadium(Iii) Reduction with Chemi-Luminescence Detection, Anal. Chem., 61, 2715–2718, 1989.
Cited by
55 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|