Author:
Liu Qingyuan,Quach Sze Ying
Abstract
Zucker Diabtic Fatty rats is a common model used for the studies related to type 2 diabetes. Metabolomics, proven by previous studies, can be used for the prediction of phenotype. This investigation held untargeted metabolomics analysis on the serum metabolites of the Zucker Diabetic Fatty rats, intended to gain insights in the study of the pathology of type 2 diabetes. LCMS method (liquid chromatography coupled with mass spectrometry) is an analytical chemistry technique used to separate and purify complex mixtures. After identifying metabolites using MS2 library spectrum matching, this investigation used Metabo Analyst to compare fatty rats and lean rats. Lastly, by applying over representation analysis, we identified seven metabolic pathways that are important for humans, which assists in discovery of therapeutic targets and the developments of diabetes biomarkers.
Reference26 articles.
1. Tanaka Y.; Gleason C. E.; Tran P. O. T.; Harmon J. S.; Robertson R. P. Prevention of Glucose Toxicity in HIT-T15 Cells and Zucker Diabetic Fatty Rats by Antioxidants. Proc Natl Acad Sci USA 1999, 6.
2. Cardiac carbohydrate metabolism in Zucker diabetic fatty rats
3. Klein M. S.; Shearer J. Metabolomics and Type 2 Diabetes: Translating Basic Research into Clinical Application. J. Diabetes Res. 11.
4. High-throughput generation, optimization and analysis of genome-scale metabolic models
5. Szabadi K.; Pinter E.; Reglodi D.; Gabriel R. Neuropeptides, Trophic Factors, and Other Substances Providing Morphofunctional and Metabolic Protection in Experimental Models of Diabetic Retinopathy. 121.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献