Author:
Veerapandian Bhuvaneshwari,Ramiah Shanmugam Saravanan,Krishnan Srividhya,Sivaraman Subramaniyasharma,Sriariyanun Malinee,Venkatachalam Ponnusami
Abstract
Effect of feeding strategy on levan production was studied in a shake flask and a 5 L lab scale fermenter. In a shake flask, levan specific substrate yield (YP/S) increased from 0.35 g levan/g sucrose to 0.48 g levan/g sucrose with repeated batch feeding. In a 3 L reactor, levan productivity of 7.18 g/L h was obtained with the fed-batch mode of fermentation. The gel permeation chromatography results indicate that higher initial sucrose concentration under the fed-batch mode operation resulted in the formation of low molecular weight fractions (4 and 10 kDa). Thus, fed-batch fermentation favors levan production resulting in higher yield and productivity and also affects the molecular weight distribution of the biopolymer.
Cited by
1 articles.
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