Author:
Puspita Sari Meli,Lara Utama Gemilang
Abstract
Yarrowia lipolytica, a yeast species capable of producing oil or oily fatty acids, has the ability to utilize multiple carbon sources, including glycerol, acetic acid, and glucose, allows for the use of inexpensive carbon sources. Waste cooking oil can be utilized as an alternative carbon source while also there is potential in increasing the oil yield due to the presence of glycerol compounds. The study aims to explore the potential of Yarrowia lipolytica in producing lipid based bioenergy from by-product such waste cooking oils. One of the greatest challenges that will affect life is our continued reliance on fossil fuels, which are still derived from petroleum and fossils. Fuel is not only the primary source of energy that has a significant impact on every aspect, but its sustainability remains the primary concern as we search for alternative solutions that can circumvent these issues. Using yeast lipids, specifically Yarrowia lipolytica, has not been investigated, in addition to producie biodiesel, this yeast can use waste cooking oil as a growth medium and produce lipids. The third generation of biodiesel uses microorganism-produced lipids, which is new and worthy of further research to solve the problem of unsustainable and environmentally unfriendly diesel fuel. Yarrowia lipolytica's ability to accumulate lipids, produce wax esters synthase enzymes, and FAEE/FAME still have great potential.