Author:
Ruensodsai Thanagorn,Panakkal Elizabeth Jayex,Teerapornnarong Prawpan,Rodiahwati Wawat,Sriariyanun Malinee,Rattanaporn Kittipong
Abstract
Chondroitin sulfate is currently one of the bioactive compounds obtained from different natural materials with high benefit in human medical treatment and pharmaceutical. However, the natural concentration of chondroitin sulfate in high cost raw material is low and this situation makes the commercialization become difficult. This study focused on production of chondroitin sulfate isolated from Bohadschia argus using enzyme-assisted extraction methodology. Optimization experiments were conducted based on Response Surface Methodology (RSM) using Box–Behnken design (BBD). Three important extraction parameters, including enzyme concentration (X1: 0.05-0.5 %), Time (X2: 0.25–3 h), and temperature (X3: 55–65 °C) were varied to obtain maximum chondroitin sulfate yields. The RSM optimized model obtained from statistical analysis presented the high correlation coefficiency (R2) at 0.7508, advocating the significance of the model. The optimum extraction conditions were selected as enzyme concentration of 0.48% with extraction time of 1.01 h. at an extraction temperature of 56.53°C. Under these conditions, the experimental yield of chondroitin sulfate was 415.59 mg/100g dry, which is in good agreement with the value predicted by the model.
Reference38 articles.
1. Free radicals, antioxidants and functional foods: Impact on human health
2. Intermittent treatment of knee osteoarthritis with oral chondroitin sulfate: a one-year, randomized, double-blind, multicenter study versus placebo11Principal Investigators: D. Uebelhart, MD, Assistant Professor; M. Malaise, MD, Professor, R. Marcolongo, MD, Professor; E. Vignon, MD, Professor. Co-Investigators: M. Piperno, MD, Head Physician; E. Mailleux, MD, Head Physician; A. Fioravanti, MD, Head Physician; L. Matoso, MD, Physician; Statistical analysis: F. DeVathaire, PhD, Professor; Radiological analysis: E. Vignon, MD, Professor.
3. High-Value Components and Bioactives from Sea Cucumbers for Functional Foods—A Review
4. yu C.C., yan W.Y., Hua D.Z., Hong C. Jun S., Optimization of microwave-assisted extraction of chondroitin sulfate from tilapia byproduct by r esponse surface methodology, (International Conference on Consumer Electronics, Communications and Networks, 2011)
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献