Research of Dracocephalum palmatum S. and Dracocephalum ruyschiana L. originating from Yakutia and identification of metabolites by tandem mass spectrometry

Abstract

Dracocephalum palmatum Stephan and Dracocephalum ruyschiana L. contains a large number of target analytes, which are biologically active compounds. High performance liquid chromatography (HPLC) in combination with a BRUKER DALTONIKS ion trap (tandem mass spectrometry) was used to identify target analytes in extracts of D. palmatum Stephan and D. ruyschiana L., originating from Yakutia. The results of initial studies revealed the presence of 61 compounds, of which 53 were identified for the first time in genus Dracocephalum. These are flavones: Apigenin 8-C-pentoside-6-C-hexoside, Apigenin 7-sulfate; Chrysin 6-C-glucoside, Chrysin glucuronide; flavanols: Kaempferol, Dihydrokaempferol, Astragalin; flavan-3-ol (epi)Catechin, phenolic acids: Methylgallic acid; Hydroxy methoxy dimethylbenzoic acid; Ellagic acid; Caffeoylshikimic acid; Prolithospermic acid; 3,4-O-dicaffeoylquinic acid; salvianolic acid G; stilbenes pinosylvin and resveratrol; anthocyanins Petunidin, Pelargonidin-3-O-glucoside; Peonidin-3-O-glucoside; Cyanidin 3-(acetyl)hexose; perillic acid; lignans: Hinokinin, Dimethyl-secoisolariciresinol, Podophyllotoxin, carotenoids: Apocarotenal, 5,8-epoxy-alpha-carotene; etc.