The SNP (g.198655287 G>A) of AHSG gene polymorphism and its association with mineral composition in Indonesia lamb meat

Abstract

The AHSG (Alpha2-Heremans-Schmid Glycoprotein) gene is indicated to have an essential role in mineral composition. This study aimed to identify the polymorphism of the AHSG gene and its association with the mineral composition of Indonesian lamb meat. The samples were 85 rams Indonesian sheep, consisting of 70 Javanese thin-tailed sheep (JTTS) and 15 Jonggol sheep (JS). AHSG gene polymorphism was analyzed using the PCR-RFLP technique with the EagI restriction enzyme. The AHSG gene association was analyzed using the T-test. The Hardy-Weinberg equilibrium analysis showed that the AHSG was polymorphic with three genotypes, i.e., GG, GA, and AA. The AHSG gene with mineral composition was associated with significance (P<0.05) on the content of Fe (iron), Zn (Zinc), and Se (Selenium). The AA genotype is the recommended genotype due to its high mineral composition. The AHSG gene (SNP g.198655287 G>A) can be used as a genetic marker for the molecular-based selection of lamb with high mineral composition.