Affiliation:
1. Hunan University of Science and Technology
Abstract
A simple and accurate method for determination of hydrogen peroxide (H2O2) using carboxymethyl cellulose coated CdS quantum dots (QDs) as a fluorescence probe was established. The influence factors of the fluorescence quenching system and the optimum conditions were investigated for analysis of hydrogen peroxide. It was found that the maximum relative fluorescence quenching intensity produced at pH 8.5 in 0.035 mol/L KH2PO4-Na2HPO4, when the concentration of CdS QDs was 1.9×10-3 mol/L and the reacting time and temperature were 45 minutes and 30°C, respectively. Under the optimum conditions, the relative fluorescence quenching intensity has a linear relationship with the logarithmic concentration of H2O2 in the range from 3×10-5 to 5×10-2 mol/L. The limit of the detection is 2.3×10-6 mol/L for H2O2. The method was used to determinate the amount of remained H2O2 in milk and Calcium Tablets successfully.
Publisher
Trans Tech Publications, Ltd.
Reference15 articles.
1. O. S. Wolfbeis, A. Durkop, M. Wu and Z. H. Lin, A europium-ion based luminescent sensing probe for hydrogen peroxide, Angew. Chem. Int. Ed, vol. 41, no. 23, p.4495–4498, (2002).
2. K. A. Fahnrich, M. Prawda and G. G. Guilbault, Recent applications of electrogenerated chemiluminescence in chemical analysis, Talanta, vol. 54, no. 4, pp.531-559, (2001).
3. M. BruchezJr, M. Moronne, P. Gin, S. Weiss and A. P. Alivisatos, Semiconductor nanocrystals as fluorescent biological labels, Science, vol. 281, no. 5385, pp.2013-2016, (1998).
4. R. Yang and S. P. Liu, Development of some molecular spectral analytical methods for the determination of proteins, Chin. J. Anal. Chem., vol. 29, no. 2, pp.232-24129, (2001).
5. H. Mattoussi, J. M. Manro, E. R. Goldman, G. P. Anderson, V. C. Sundar, F. V. Mikula, et al,. Self-assembly of CdSe−ZnS quantum dot bioconjugates using an engineered recombinant protein, J. Am. Chem. Soc., vol. 122, no. 49, pp.12142-12150 , (2000).
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献