Affiliation:
1. Hebei North University
Abstract
AIMS: To explore gene expressions of Bax and changes of cytC releasing in the CIR hippocampus neuron; to observe the effect on CIR with ligustrazine. METHODS: According to Bannisters, we made ischemia and reperfusion models of rat, and then using ligustrazine to cure; we observed the expression changes of Bax gene in hippocampus neurons by nucleic acid in situ hybridization, detecting cytC release through Western blotting. RESULTS: Ligustrazine protect neuron by reducing neuronal apoptosis induced by CIR, and inhibiting release of cytc by releasing.
Publisher
Trans Tech Publications, Ltd.
Reference15 articles.
1. Fu XC, Xu Z, He Y, et al. Protective function of Huanglian Jiedu Tang on Rats after focal cerebral ischemic reperfusion and its influence on caspase-3 expression [J]. Modern Journal of Integrated Chinese Traditional and Western Medicine, 2009, 18(14): 1598-9.
2. Zhao QZ, Li W, Bo AH, et al. Neuron Ulstructure and Protective Effects of Ligustrazine in Rats Cortex after Cerebral Ischemia Reperfusion[J]. Lishizhen Medicine and Materia Medica Research, 2009, 20(3): 683-4.
3. Li J, Han B, Ma X, et al. The effects of propofol on hippocampal caspase-3 and Bcl-2 expression following forebrain ischemia-reperfusion in rats[J]. Brain Res, 2010, 1356: 11-23.
4. Ren D, Kim H, Tu HC, et al. The VDAC2-BAK rheostat controls thymocyte survival[J]. Sci Signal, 2009, 2(85): a48.
5. Raisova M, Hossini AM, Eberle J, et al. The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis[J]. J Invest Dermatol, 2001, 117(2): 333-40.