The Effect of Glycerol Concentration on Biocomposite Bacterial Cellulose-Chitosan Characterization as Dura Mater Artificial

Abstract

Bacterial cellulose and chitosan have been widely developed for biomaterial applications, one of which is used as a dura mater artificial. In designing dura mater artificial, there are several criteria that must be met, one of which is mechanical that can be seen through tensile strength and elongation value. In previous study, the mechanical properties of biocomposite bacterial cellulose-chitosan still too rigid and did not meet the standard. This research was conducted to determine the effect of the addition of glycerol concentration to the physical and biological of bacterial cellulose-chitosan membrane. Bacterial cellulose membranes with the addition of glycerol concentration of 0%; 0,25%; 0,5% and 0,75% were dried with oven and immersed for 6 hours in 0.5% chitosan solution. Characterization was performed by functional group, morphology, tensile strength, swelling, degradation and cytotoxicity test. Based on the results, it can conclude that biocomposite bacterial cellulose-chitosan-glycerol showed suitable characteristics as a dura mater artificial.