Phase Separation of Reverse Micelles

Abstract

his paper describes the rules of change of reverse micelle phase separation under acid conditions. Using acetic acid, the reverse micelle system can be separated into an upper and lower phase. After separation with acid, the continuous phase is an organic solution and the polarity of upper phase is lower than that of lower layer. The separation formula is y=1.62+20.84e-x/5.31, where x is the W0 value of Superscript textthe original micelle system, y is the concentration of acetic acid required to separate the reverse micelle system into two phases of equal volume and it can be used to check the formula. Applying this formula to calculate the concentration of acid required to affect separation, the protein of rat serum can be separated under acidic conditions by the reverse micelle extraction method. In this way serum protein of rat can be further separated.