Comparing the Properties of Relatively High Concentration LPA by Capillary Electrophoresis-Reference-Cited by-同舟云学术

Comparing the Properties of Relatively High Concentration LPA by Capillary Electrophoresis

Published:2013-12 Issue: Volume:850-851 Page:1283-1286
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Wang Jie¹,Wan Wei¹

Affiliation:

1. GuangDong Polytechnic Normal University

Abstract

Two relatively high concentration 4% and 6% the replaceable Linear polyacrylamide (LPA) gel was used as the sieving medium. An extensive series of experiments has been performed using DNA fragments ranging in size from 80 to 587 base pairs; the properties such as the mobilities, the width at half height, and the resolution were compared. The electric field was changed from 100V/cm to 375V/cm. From the experiment results, it is shown: increasing the gel concentration from 4%LPA to 6%LPA can not improve the resolution to separate the DNA sample (the length of fragments600bp). This work complements our systematic study and will help to further optimize and improve high performance DNA separation in microfluidic applications.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.850-851.1283.pdf

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