An Innovative Method on the Polyphosphate Determination in Aquatic Product with Interdiction of Polyphosphate Hydrolysis by Using Ion Chromatography

Abstract

Trichloracetic acid (TCA) was used to denature the enzymes that hydrolyze polyphosphates in frozen aquatic product and the polyphosphates were determined by using ion chromatograph with suppressor conductivity. The results showed that TCA could block the enzymatic hydrolysis of pyrophosphate and tripolyphosphate. The instrument was Dionex ICS 2000 ion chromatograph. The KOH solution was used as the. Ionpac AS11-HC separator column with gradient elution by KOH solution and suppressed conductivity detector were used for determine orthophosphate (Pi), pyrophosphate (PP), tripolyphosphate (TPP), and hexametaphosphte (HMP). The method was linear at concentration raging from 0.005 mmol•L-1 to 0.5mmol•L-1. The average recoveries ranged from 99% to 111%. The method can be applied for determination of phosphates in aquatic products.