Solid-State Fermentation Production of α-Galactosidase by Aspergillus niger Zju-Y1

Abstract

The agro-byproduct culture medium for α-galactosidase production by Aspergillus niger zju-Y1 was optimized with flask-SSF via response surface methodology, maximum α-galactosidase yield at the level of 230.159 U/g dry matter was achieved by using wheat bran and soybean meal as culture medium components. Furthermore, based on the flask-SSF results, the three-section-control strategy was developed for scale-up SSF, making fermentation steadily and efficiently, and the α-galactosidase activity hitting to 174.410 U/g dry matter, which is close to the result of flask-SSF. The results demonstrated that a feasible scale-up SSF mode for α-galactosidase production is successfully set up by combining the utilization of agro-byproduct culture medium and three-section-control strategy.