Biogrowth of Escherichia coli in Bio-Ionic Media

Abstract

The impact of development of ionic liquids (ILs) in biochemical appliances has attracted attention from many researchers to further investigate on the potential of ILs. Use of ILs has provided an effective alternative in the conversion of source of carbohydrate in woody plant into fermentable sugar for ethanol production. To investigate how the presence of ILs affects the fermentation process, fermentation by using E. coli were conducted in different fermentation conditions with the presence of ILs. The purpose of this research is to investigate microbial growth under the presence of ILs with various parameters. Ability of E. coli to grow in facultative condition has made these bacteria suitable for this research. In this research, the growths of E. coli in the presence of ILs were observed by shaken culture method for 24 hours. The E. coli was tested to grow in 5 % v/v [0.005, 20 % v/v [0.02, and 50 % v/v [0.05 of IL concentration ratios. The three types of ILs used for this research were 1-Ethyl-3-methylimidazolium Acetate [EMIM][A, 1-Butyl-3-methylimidazolium Chloride [BMIM][Cl] and 1-Allyl-3-methylimidazolium Chloride [AMIM][Cl]. The growth patterns of E. coli were also observed during the fermentation with shaking frequency of 250 rpm, 300 rpm and 350 rpm.