Synthesis of Vitamin B<sub>12</sub> Artificial Antigen and Determination of Hapten Coupling Ratio-Reference-Cited by-同舟云学术

Synthesis of Vitamin B₁₂ Artificial Antigen and Determination of Hapten Coupling Ratio

Published:2014-06 Issue: Volume:955-959 Page:1009-1012
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Yang Fan Fan¹,Zhu Li Xin¹,Liu Ren Rong¹,Xu Long¹,Wei Meng¹

Affiliation:

1. Jiangxi Science ＆Technology Normal University

Abstract

Vitamin B12(vB12) was coupled to cBSA by N,N'-Carbonyldiimidazole (CDI) method. The result was confirmed by UV spectrum analysis. It showed that vB12conjugates (vB12-cBSA) were successfully synthesized. By using UV scanning the coupling ratio of artificial antigen was determined which was 6:1. The successful synthesis of vitamin B12artificial antigen is important to establish immunoassay for determining vitamin B12

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.955-959.1009.pdf

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