Response Surface Methodology for Extraction Optimization of Phyllostachys pubescens Bamboo Shoots Protein

Author:

Lv Guo Ti1,Li Ji Hua1,Huang Chang Xin2,Zhu Bing Qing1,Huang Xiao Bing3,Lin Li Jing1,Xiong Shan Bai4

Affiliation:

1. Chinese Academy of Tropical Agricultural Sciences

2. Zhanjiang Normal University

3. Agricultural Product Processing Research Institute of Chinese Academy of Tropical Agricultural Sciences

4. Huazhong Agricultural University

Abstract

The aim of the study was to investigate bamboo shoots protein extraction. A Box-Behnken design (BBD) was applied through the Response Surface Methodology (RSM) to determine the effects of pH, temperature and solid-liquid ratio during Phyllostachys pubescens bamboo shoots protein extraction. Regression analysis was applied on the data obtained. The more relevant variables were pH, liquid-solid ratio. The coefficient determination (R2 = 0.9258) was good for the second-order model. Maximum yield was 10.77±2.28% obtained when pH, liquid-solid ratio, temperature and extraction time were 12, 32.24, 46.12°C and 20 min, respectively. The result was help to design the process of optimal protein extraction from Phyllostachys pubescens bamboo shoots.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

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