The Antioxidation Activity In Vivo and In Vitro of Flavonoids and Chlorogenic Acid Isolated from Eucommia ulmoides Leaves

Abstract

The flavonoids and chlorogenic acid were separated and purified from Eulmoids ulmoids leaves, and their antioxidation activity in vivo and in vitro was investigated by animal test and free radical scavenging test. The result shows that Eucommia ulmoides leaves flavones and chlorogenic acid can significantly reduce the content of malondialchehyche (MDA) (p<0.05), but only Eucommia ulmoides leaves chlorogenic acid can significantly enhance the activity of superoxide dismutase (SOD) (p<0.05) and glutathione peroxidase (GSH-PX) (p<0.05), which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vivo; both Eucommia ulmoides Leaves flavones and chlorogenic acid have good capacity on scavenging hydroxyl radical (HO•), superoxide anion radical (O2-•) and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH•), and show good doses-response relationship, but Eucommia ulmoides leaves chlorogenic acid shows highest scavenging rate on every radical, which means that Eucommia ulmoides leaves chlorogenic acid has more significant antioxidation activity in vitro.