Hydrogen-Terminated Diamond Sensors for Electrical Monitoring of Cells

Author:

Izak Tibor1,Novotná Katarína2,Kopová Ivana2,Bačáková Lucie2,Varga Marián1,Rezek Bohuslav1,Kromka Alexander1

Affiliation:

1. Academy of Sciences of Czech Republic

2. Academy of Sciences of the Czech Republic

Abstract

In this paper we introduce fully optically transparent impedance biosensors based on intrinsic nanocrystalline diamond (NCD) films deposited on glass substrate. Prepared sensors have an interdigital electrode (IDE) structures realized by local hydrogen and oxygen termination of diamond surface, which mean in-plane configuration of active sensor area. Sensors were tested by real time monitoring of human osteoblast-like MG 63 cells in wide frequency range from 10 Hz to 100 kHz for several days. Two different measurement setups were used and compared regarding to their advantages and disadvantages. Proof of concept of diamond-based impedance sensor is showed, i.e. time dependence and frequency dependence (Nyquist plots) of absolute impedance.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Reference8 articles.

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3. Roche Applied Science: Biochemica 2 (2009), p.18.

4. T. Izak, O. Babchenko, M. Varga, S. Potocky, and A. Kromka: phys. status solidi b 249 (2012) 2600.

5. R.J. Hamers, J.E. Butler, T. Lasseter, B.M. Nichols, J.N. Russell, K.Y. Tse, and W. Yang: Diamond and Related Materials 14 (2005), p.661–668.

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