A Hybrid-Structured Microfluidic Chip Developed for ATP Bioluminescence Detection

Author:

Zhang Wu Ming1,Li Zhen Yu1,Chen Tao1,Yao Jian Jiang1,Lv Yang1,Li Min1,Li Guang2

Affiliation:

1. Zhenjiang University

2. Zhejiang University

Abstract

A hybrid-structured microfluidic chip integrating optical fiber sensing technology and bioluminescence chemical reaction mechanism was developed for rapid and sensitive detection of adenosine 5’-triphosphate (ATP). This microfluidic chip was fabricated with five distinct layers for the successive steps of sample-enzyme reagent mixing, bioluminescence chemical reaction and scatting light collection. The experimental results demonstrated that the provided hybrid-structure improved the sensitivity, response time, reagent consumption and other properties of chip. This improvement enables the microfluidic chip to attain a sensitive ATP detection in the range from 10-9to 10-4M with excellent linearity (R2= 0.9967), low detection error (CV < 15%), and tiny reagent consumption (< 20 μL). These achievements thus indicated that the chip developed in this study possess the advantages such as high sensitivity, quick-response and small reagent consumption.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

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