Anti Wnt-1 Monoclonal Antibody’s Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines

Abstract

Wnt-β Catenin pathway has an important role in many cancers. Wnt-1 protein from the Wnt protein family, which regulates this pathway, has a special effect on the development of breast cancer. Monoclonal antibodies attaching to metal nanoparticles have an important role in the diagnosis and treatment of cancers.In this study, the anti-Wnt-1monoclonal antibody was conjugated to the gold nanoparticles synthesized by theTurkevichmethod. Conjugation was achieved using the EDC-NHS method. The density of the monoclonal antibodies bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 and MDA-MB-231 breast cancer cell lines were treated with conjugated nanoparticles for 48 h, followed by performing a double-staining method to detect apoptosis cells.The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles to have a positive effect on Anti-Wnt-1monoclonal antibodies, leading to an increase in the number of conjugating nanoparticles.