Affiliation:
1. Northeast Agricultural University
Abstract
This experiment contrasted the hydrolytic effect of SPI among the six different proteolytic ferments and the Alcalase basic protein which can produce the best antioxygen peptide, the optimization technology condition of hydrolytic SPI determined by orthogonally experimentation: concentration of substrate 7.0%, the dozes of enzyme 9000U/g substrate, temperature 52.5°C, pH 8.5, the reaction time 5h. The antioxygen activity of hydrolysis product is researched from the capability of cleaning OH·, the capability of cleaning O2-· and the capability of inhibiting ROO·. After hydrolyze of 5h, the OH· inhibition ratio of the production is 36.43%, the activity of cleaning O2-· of the production is 317.73U/g, the ROO· inhibition ratio of the production is 46.24%.
Publisher
Trans Tech Publications, Ltd.
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