Affiliation:
1. N.V. Vereshchagin Vologda State Dairy Farming Academy
2. All-Russian Research Institute for Silviculture and Mechanizati on of Forestry
3. Kostroma State Agricultural Academy
4. All-Russian Research Institute for Silviculture and Mechanization of Forestry
Abstract
Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots.
Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilization time on the viability of honeysuckle explants at the stage of culture introduction in vitro. The effect of the growth regulator Cytodef in the QL nutrient medium on organogenesis was studied at the stage of micropropagation proper, the effect of auxin IBA on plant root formation – at the stage of rooting in vitro.
Results and discussion. The greatest viability of honeysuckle explants (80–94%) was registered in the samples affected by Lizoformin 3000 (5%) and silver nitrate (0.2%) as sterilizing agents with a sterilization time of 10 min at the stage of in vitro culture introduction. The biggest quantity (8.8 pcs.) and total length (40.1 cm) of microshoots were observed when the content of cytokinin Cytodef in the culture medium QL was 0.3 mg/L at the stage micropropagation proper. The Boreal Beast cultivar had the largest total length of shoots (29.0 cm). The biggest quantity (5.5 pcs.) and total length (30.8 cm) of roots resulted from 0.5 mg/L of auxin IBA at the stage of rooting in vitro. Coconut substrate produced the highest survival rate (92–99%) at the stage of adaptation to non-sterile conditions in vivo, with the greatest number of leaves (8.1–10.2 pcs.) observed in Canadian cultivars.
Conclusion. Cytodef and IBA proved to be effective growth-regulating substances for microplants of Russian and Canadian honeysuckle cultivars in vitro, which makes them promising for berry plantations.
Publisher
Kemerovo State University
Subject
Industrial and Manufacturing Engineering,Economics, Econometrics and Finance (miscellaneous),Food Science
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