Performance and robustness analysis reveals phenotypic trade-offs in yeast

Author:

Trivellin Cecilia1ORCID,Rugbjerg Peter12ORCID,Olsson Lisbeth1ORCID

Affiliation:

1. Department of Life Sciences, Division of Industrial Biotechnology, Chalmers University of Technology

2. Enduro Genetics ApS, Copenhagen, Denmark

Abstract

To design strains that can function efficiently in complex industrial settings, it is crucial to consider their robustness, that is, the stability of their performance when faced with perturbations. In the present study, we cultivated 24Saccharomyces cerevisiaestrains under conditions that simulated perturbations encountered during lignocellulosic bioethanol production, and assessed the performance and robustness of multiple phenotypes simultaneously. The observed negative correlations confirmed a trade-off between performance and robustness of ethanol yield, biomass yield, and cell dry weight. Conversely, the specific growth rate performance positively correlated with the robustness, presumably because of evolutionary selection for robust, fast-growing cells. The Ethanol Red strain exhibited both high performance and robustness, making it a good candidate for bioproduction in the tested perturbation space. Our results experimentally map the robustness–performance trade-offs, previously demonstrated mainly by single-phenotype and computational studies.

Funder

Novo Nordisk Fonden

Publisher

Life Science Alliance, LLC

Subject

Health, Toxicology and Mutagenesis,Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology

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