Cytokines regulate membrane adenosine deaminase on human activated lymphocytes

Author:

Cordero Oscar J1,Salgado Francisco J1,Fernández-Alonso Carmen M1,Herrera Carolina2,Lluis Carmen2,Franco Rafael2,Nogueira Montserrat1

Affiliation:

1. Departments of Biochemistry and Molecular Biology, University of Santiago de Compostela , 15706 Santiago de Compostela

2. Departments of Biochemistry and Molecular Biology, University of Barcelona , 08108 Barcelona, Spain

Abstract

Abstract CD26 is a lymphocyte marker that can anchor adenosine deaminase (ADA) on the T cell surface. We found that ADA is regulated by cytokines on the cell surface during T cell activation. By means of flow cytometry, immunofluorescence, and immunoblotting techniques, we found that interleukin (IL)-2 and IL-12 up-regulate ecto-ADA and CD26 expression. In clear contrast, IL-4 led to down-regulation of lymphocyte surface ADA without modifying the level of CD26. Moreover, neither circulating ADA transcription nor mRNA translation was regulated by cytokines. These results, along with absence of total-ADA modulation, the variable amount of ADA found in purified plasma membranes, and the different effect of Brefeldin A on the surface presence of ADA and CD26 indicated that cytokines regulate the translocation of ADA towards the cell surface through a mechanism not involving CD26. Ecto-ADA protected activated lymphocytes from the toxic effects of extracellular adenosine. Therefore, this cell surface ADA control might constitute part of the fine immunoregulatory mechanism of adenosine-mediated signaling through purinergic receptors in leukocytes.

Funder

Xunta de Galicia

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Immunology,Immunology and Allergy

Reference41 articles.

1. Interference with thymocyte differentiation by an inhibitor of N-adenosylhomocysteine hydrolase;Benveniste;J. Immunol.,1995

2. Adenosine deaminase-deficient mice generated using a two-stage genetic engineering strategy exhibit a combined immunodeficiency;Blackburn;J. Biol. Chem.,1998

3. Adult onset immunodeficiency caused by inherited adenosine deaminase deficiency;Shovlin;J. Immunol.,1994

4. Serum adenosine deaminase: isoenzymes and diagnostic application;Ungerer;Clin. Chem.,1992

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3