ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF MAJOR FLAVONOID IN LEAVES OF PERESKIA BLEO (KUNTH) DC

Abstract

Objective: This study aimed to isolate, identify, and quantify major flavonoid in P. bleo leaves from West Java Regency, Indonesia. Methods: Isolation began with maceration, followed by liquid-liquid extraction and various chromatographic separations. Identification of isolates was carried out using 2D TLC and shifting reagents. Quantification was determined with the colorimetric method. Results: The results showed that extract, ethyl acetate, and distilled water fractions contain flavonoids. The flavonoid screening showed positive results for flavonols. The vacuum liquid chromatography separated 21 sub-fractions; the 12th and 13th sub-fractions were predicted to contain flavonols. Conclusion: The novelty of this study was the isolation and identification of the major flavonoid of P. bleo leaves originating from West Java Regency, Indonesia, i.e. catechin. The content of the compound was 3.795±0.096 g QE/ml with a purity of 94.89%.