One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation-Reference-Cited by-同舟云学术

One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

Published:2018-09-04 Issue:10 Volume:215 Page:2686-2695
ISSN:0022-1007
Container-title:Journal of Experimental Medicine
language:en
Short-container-title:

Author:

Jacobsen Johanne T.¹²,Mesin Luka¹,Markoulaki Styliani³,Schiepers Ariën¹^ORCID,Cavazzoni Cecília B.¹⁴,Bousbaine Djenet⁵,Jaenisch Rudolf³,Victora Gabriel D.¹^ORCID

Affiliation:

1. Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY

2. Center for Immune Regulation, Oslo University Hospital, University of Oslo, Oslo, Norway

3. Whitehead Institute for Biomedical Research, Cambridge, MA

4. Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

5. Boston Children’s Hospital, Harvard Medical School, Boston, MA

Abstract

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.

Funder

National Institute of Allergy and Infectious Diseases

Searle Scholars Program

Research Council of Norway

Seventh Framework Programme

CAPES

Boehringer Ingelheim Fonds

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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