Abstract
The possibility of using various methods for determining the viability of cultures of microalgae Dunaliella salina and Chlorococcum dissectum before and after freezing-warming was investigated and analyzed. It has been established that the selection of an effective method should be carried out individually for each culture. For an integral assessment of the proliferative and metabolic activity of cells of both species of the studied microalgae, Alamar Blue-test and the ability to grow on liquid nutrient media can be used. The use of the Koch plate method, MTT-test and TTC staining is possible only for the microalga C. dissectum. Vital staining with trypan blue was found to be incorrect.
Publisher
National Academy of Sciences of Ukraine (Co. LTD Ukrinformnauka) (Publications)
Reference25 articles.
1. Ahmed S.А., Gogal R.M., Walsh J.E. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to 3H-thymidine incorporation assay. J. Immunol. Meth. 2(170): 211-224.
2. Al-Rikabey M.N., Al-Mayah A.M. 2018. Cultivation of Chlorella vulgaris in BG-11 Media Using Taguchi Method. J. Adv Res. Dynamic. Control Syst. 10(7): 19-30.
3. Anikina L., Puchov S., Dubrovskaya S., Afanasyeva S., Klochkov S. 2014. Comparative determination of cell viability using MTT and resazurin. Fund. Res. 7(12): 1423-1427.
4. Aravantinou A.F., Manariotis I.D. 2016. Effect of operating conditions on Chlorococcum sp. growth and lipid production. J. Environ. Chem. Eng. 1(4): 1217-1223. https://doi.org/10.1016/j.jece.2016.01.028
5. Bonnier F., Keating M.E., Wróbel T.P., Majzner K., Baranska M., Garcia-Munoz A., Blanco A., Byrne H.J. 2015. Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models. Toxicol. in Vitro. 29: 124-131. https://doi.org/10.1016/j.tiv.2014.09.014
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献