Simultaneous Spectrophotometric Determination of Lamivudine and
Stavudine using π-Acceptors as Analytical Reagents
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Published:2020
Issue:3
Volume:5
Page:234-241
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ISSN:2456-8937
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Container-title:Asian Journal of Organic & Medicinal Chemistry
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language:en
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Short-container-title:Asian J. Org. Med. Chem.
Author:
Sayanna 1, Veeraiah T.1, Ramana Reddy Ch. Venkata2
Affiliation:
1. Department of Chemistry SAP College, Vikarabad-501101, India 2. Department of Chemistry, JNTUH College of Engineering, Kukatpally, Hyderabad-500085, India
Abstract
Two new sensitive and precise spectrophotometric methods have been proposed and developed for the
simultaneous estimation of lamivudine and stavudine in pure mixture and in pharmaceutical binary
dosage forms. A new concept of area under curve (AUC) is proposed for simultaneous estimation of
two drugs by these methods. Method A involves the use of 2,3-dichloro-5,6-dicyano-1,4-benzoquinone
(DDQ) as analytical reagent and the AUC between 390 nm and 690 nm for DDQ was used for
determination. Method B involves the use of p-chloranilic acid: 2,5-dichloro-3,6-dihydroxy-1,4-
benzoquinone (p-CA) as an analytical reagent and the AUC between 400 and 700 nm for p-chloranilic
acid was used for deter-mination. The methods developed and construction of calibration curves using
two analytical reagents viz., DDQ and p-chloranilic acid are described. Optical and analytical parameters
for the individual and simultaneous determination of lamivudine and stavudine using AUC are tabulated.
The methods have been validated and compared with HPLC methods in terms of standard deviation,
t-test and F-test.
Publisher
Asian Journal of Chemistry
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