Laccase Enzyme Catalyst Mediated Synthesis of Pyrimidines and its Antibacterial, Antifungal, Molecular Docking Studies

Abstract

The novel synthesis of pyrimidine derivatives (1a-f) by using green chemistry technique with laccase as an enzyme catalyst. FT-IR, 1H NMR, and elemental analyses were used to describe the produced compounds (1a-f). Antibacterial, antifungal, and molecular docking investigations to characterize the produced pyrimidine derivatives (1a-f). In antibacterial testing, compound 1e, showed considerable action towards E. coli (MIC 02 μg/mL) as associated to ciprofloxacin (MIC 04 μg/mL). When related to clotrimazole in antifungal assessment, compound 1d was shown to be significant activity towards C. albicans (MIC 0.25 μg/mL) than clotrimazole (MIC 0.5 μg/mL). Molecular docking experiments further indicate that compound 1e inhibited antibacterial and compound 1d inhibited antifungal proteins more effectively than the control compounds ciprofloxacin and clotrimazole.