Transcriptome profiling of kenaf (Hibiscus cannabinus L.) under plumbic stress conditions implies the involvement of NAC transcription factors regulating reactive oxygen species-dependent programmed cell death

Abstract

Heavy metal contamination of soils has become a serious global issue, and bioremediation has been proposed as a potential solution. Kenaf (Hibiscus cannabinus L.) is a fast growing, non-woody multipurpose annual plant that is suitable for removing excess heavy metals from soils. However, there has been relatively little research on the kenaf molecular mechanisms induced in response to an exposure to heavy metal stress. Thus, whole kenaf seedlings grown under control (normal) and stress (plumbic treatment) conditions were sampled for transcriptome sequencing. Unigenes generated through the de novo assembly of clean reads were functionally annotated based on seven databases. Transcription factor (TF)-coding genes were predicted and the physiological traits of the seedlings were analyzed. A total of 44.57 Gb high-quality sequencing data were obtained, which were assembled into 136,854 unigenes. These unigenes included 1,697 that were regarded as differentially expressed genes (DEGs). A GO enrichment analysis of the DEGs indicated that many of them are related to catalytic activities. Moreover, the DEGs appeared to suggest that numerous KEGG pathways are suppressed (e.g., the photosynthesis-involving pathways) or enhanced (like the flavonoid metabolism pathways) in response to Pb stress. Of the 2,066 predicted TF-coding genes, only 55 were differentially expressed between the control and stressed samples. Further analyses suggested that the plumbic stress treatment induced reactive oxygen species-dependent programmed cell death in the kenaf plants via a process that may be regulated by the differentially expressed NAC TF genes.