Development of Expressed Sequence Tag–Simple Sequence Repeat Markers Related to the Salt-Stress Response of Kenaf (Hibiscus cannabinus)

Author:

An Xia1ORCID,Liu Qin12,Ying Jinyao3,Wei Jiqian4,Dong Guoyun5,Luo Xiahong1,Li Wenlue1,Liu Tingting1,Zhou Huaping3,Zou Lina1,Chen Changli1

Affiliation:

1. Zhejiang Xiaoshan Institute of Cotton & Bast Fiber Crops, Zhejiang Institute of Landscape Plants and Flowers, Zhejiang Academy of Agricultural Sciences, Hangzhou 311251, China

2. Institute of Resource Plants, Yunnan University, Kunming 650500, China

3. Hangzhou Xiaoshan District Agricultural (Forestry) Technology Promotion, Hangzhou 311203, China

4. Hangzhou Agricultural Technology Extension Center, Hangzhou 310020, China

5. Zhangjiajie Research Institute of Agricultural Science and Technology, Zhangjiajie 427000, China

Abstract

Kenaf is one of the most important natural cannabis plants. Molecular marker-assisted breeding is vital for accelerating the breeding process of kenaf. However, the number of kenaf markers is insufficient for molecular marker-assisted breeding. Using transcriptome sequencing data for salt-stressed kenaf plants, the number and distribution of simple sequence repeats (SSRs) and single nucleotide variations (SNVs) in the expressed sequences were determined. The objectives of this study were to elucidate the sequence variations in kenaf genes expressed in response to salt stress and to identify stable and dependable molecular markers. Primers were designed for SSR loci and then EST-SSR molecular markers were generated. The subsequent analyses revealed that 30.50% of the unigenes contained SSR motifs, most of which were single nucleotides followed by trinucleotides and dinucleotides. The unigenes containing SSRs were mostly associated with kenaf salt tolerance. Additionally, 10,483 SNVs were detected in contig sequences. Of the 3995 differentially expressed genes encoding interacting proteins, 1297 contained SSRs. Most of these genes were associated with metabolic pathways (e.g., 03000 transcription factors, B09132 signal transduction, and 04122 sulfur relay system). We designed 20 pairs of EST-SSR primers to genotype 30 kenaf varieties (lines), of which 9 primer pairs were ideal for genotyping (e.g., 1 highly polymorphic marker and 2 moderately polymorphic markers). The primer pairs designed for the EST-SSR markers in the kenaf genome may be useful SSR molecular markers for future research on kenaf. The verified polymorphic markers may be applicable to the molecular marker-assisted breeding of salt-tolerant kenaf varieties.

Funder

China Agriculture Research System of MOF and MARA

China Agriculture Research System for Bast and Leaf Fiber Crops

Publisher

MDPI AG

Subject

Agronomy and Crop Science

Reference39 articles.

1. Food security and climate change: On the potential to adapt global crop production by active selection to rising atmospheric carbon dioxide;Ziska;Proc. Biol. Sci.,2012

2. Measuring soil sustainability via soil resilience;Ludwig;Sci. Total Environ.,2018

3. Mapping disruption and resilience mechanisms in food systems;Savary;Food Secur.,2020

4. Comparative transcriptomic analysis reveals key genes and pathways in two different cadmium tolerance kenaf (Hibiscus cannabinus L.) cultivars;Chen;Chemosphere,2021

5. Genetic diversity of kenaf (Hibiscus cannabinus) evaluated by inter-simple sequence repeat (ISSR);Zhang;Biochem. Genet.,2013

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