Mitochondrial genomics of human pathogenic parasiteLeishmania(Viannia)panamensis

Author:

Urrea Daniel Alfonso12,Triana-Chavez Omar2,Alzate Juan F.3

Affiliation:

1. Laboratorio de Investigaciones en Parasitología Tropical (LIPT), Departamento de Biología, Facultad de Ciencias, Universidad del Tolima, Ibague, Tolima, Colombia

2. Grupo Biología y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medellín, Antioquia, Colombia

3. Centro Nacional de Secuenciación Genómica -CNSG, Sede de Investigación Universitaria -SIU. Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellin, Antioquia, Colombia

Abstract

BackgroundThe human parasiteLeishmania (V.) panamensisis one of the pathogenic species responsible for cutaneous leishmaniasis in Central and South America. Despite its importance in molecular parasitology, its mitochondrial genome, divided into minicircles and maxicircles, haven’t been described so far.MethodsUsing NGS-based sequencing (454 and ILLUMINA), and combiningde novogenome assembly and mapping strategies, we report the maxicircle kDNA annotated genome ofL. (V.) panamensis, the first reference of this molecule for the subgenusViannia. A comparative genomics approach is performed against otherLeishmaniaandTrypanosomaspecies.ResultsThe results show synteny of mitochondrial genes ofL. (V.) panamensiswith other kinetoplastids. It was also possible to identify nucleotide variants within the coding regions of the maxicircle, shared among some of them and others specific to each strain. Furthermore, we compared the minicircles kDNA sequences of two strains and the results show that the conserved and divergent regions of the minicircles exhibit strain-specific associations.

Funder

Universidad de Antioquia UdeA, and by a Ph.D. Studentship funded by Colciencias

Publisher

PeerJ

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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