Author:
Hoyos Sanchez Maria Camila,Ospina Zapata Hader Sebastian,Suarez Brayhan Dario,Ospina Carlos,Barbosa Hamilton Julian,Carranza Martinez Julio Cesar,Vallejo Gustavo Adolfo,Urrea Montes Daniel,Duitama Jorge
Abstract
AbstractChagas is an endemic disease in tropical regions of Latin America, caused by the parasite Trypanosoma cruzi. High intraspecies variability and genome complexity have been challenges to assemble high quality genomes needed for studies in evolution, population genomics, diagnosis and drug development. Here we present a chromosome-level phased assembly of a TcI T. cruzi strain (Dm25). While 29 chromosomes show a large collinearity with the assembly of the Brazil A4 strain, three chromosomes show both large heterozygosity and large divergence, compared to previous assemblies of TcI T. cruzi strains. Nucleotide and protein evolution statistics indicate that T. cruzi Marinkellei separated before the diversification of T. cruzi in the known DTUs. Interchromosomal paralogs of dispersed gene families and histones appeared before but at the same time have a more strict purifying selection, compared to other repeat families. Previously unreported large tandem arrays of protein kinases and histones were identified in this assembly. Over one million variants obtained from Illumina reads aligned to the primary assembly clearly separate the main DTUs. We expect that this new assembly will be a valuable resource for further studies on evolution and functional genomics of Trypanosomatids.
Funder
Ministerio de Ciencia, Tecnología e Innovación
Publisher
Springer Science and Business Media LLC
Reference102 articles.
1. Rassi, A. J., Rassi, A. & De Rezende, J. M. American TRYPANOSOMIASIS (Chagas Disease). Infect. Dis. Clin. N. Am. 26, 275–291. https://doi.org/10.1016/j.idc.2012.03.002 (2012).
2. World Health Organization. (2020). Chagas disease (also known as American trypanosomiasis). https://www.who.int/en/news-room/fact-sheets/detail/chagas-disease-(american-trypanosomiasis).
3. Pan American Health Organization. (2022). Chagas disease. Chagas Disease in the Americas for Public Health Workers. https://www.paho.org/en/documents/factsheet-chagas-disease-americas-public-health-workers.
4. Ministerio de Salud y Protección Social. (2013). Enfermedad de Chagas: Memorias. Bogota D.C. https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/TH/Memorias_chagas.pdf.
5. Olivera, M. J., Fory, J. A., Porras, J. F. & Buitrago, G. Prevalence of Chagas disease in Colombia: A systematic review and meta-analysis. PLoS One 14(1), e0210156. https://doi.org/10.1371/journal.pone.0210156 (2019).