Affiliation:
1. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
Abstract
Pseudomonas cannabinapv.alisalensis(Pcal), which causes bacterial blight disease of Brassicaceae, is an economically important pathogen worldwide. To identifyPcalgenes involved in pathogenesis, we conducted a screen for 1,040 individualPcalKB211 Tn5mutants with reduced virulence on cabbage plants using a dip-inoculation method. We isolated 53 reduced virulence mutants and identified several potential virulence factors involved inPcalvirulence mechanisms such as the type III secretion system, membrane transporters, transcription factors, and amino acid metabolism. Importantly,Pcalis pathogenic on a range of monocotyledonous and dicotyledonous plants. Therefore, we also carried out the inoculation test on oat plants, which are cultivated after cabbage cultivation as green manure crops. Interestingly among the 53 mutants, 31 mutants also exhibited reduced virulence on oat seedlings, indicating thatPcaloptimizes its virulence factors for pathogenicity on different host plants. Our results highlight the importance of revealing the virulence factors for each plant host-bacterial interaction, and will provide new insights intoPcalvirulence mechanisms.
Funder
JSPS KAKENHI
JST ERATO NOMURA Microbial Community Control Project, JST, Japan
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience
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