Resequencing and characterization of the first Corynebacterium pseudotuberculosis genome isolated from camel

Author:

Giovanelli Tacconi Gimenez Enrico1,Viana Marcus Vinicius Canário1,de Jesus Sousa Thiago2,Aburjaile Flávia3,Brenig Bertram4,Silva Artur5,Azevedo Vasco1

Affiliation:

1. Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

2. Laboratório Central do Espírito Santo (LACEN-ES), Vitória, Espírito Santo, Brazil

3. Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

4. Institute of Veterinary Medicine, University of Göttingen, Göttingen, Niedersachsen, Germany

5. Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil

Abstract

Background Corynebacterium pseudotuberculosis is a zoonotic Gram-positive bacterial pathogen known to cause different diseases in many mammals, including lymph node abscesses in camels. Strains from biovars equi and ovis of C. pseudotuberculosis can infect camels. Comparative genomics could help to identify features related to host adaptation, and currently strain Cp162 from biovar equi is the only one from camel with a sequenced genome. Methods In this work, we compared the quality of three genome assemblies of strain Cp162 that used data from the DNA sequencing platforms SOLiD v3 Plus, IonTorrent PGM, and Illumina HiSeq 2500 with an optical map and investigate the unique features of this strain. For this purpose, we applied comparative genomic analysis on the different Cp162 genome assembly versions and included other 129 genomes from the same species. Results Since the first version of the genome, there was an increase of 88 Kbp and 121 protein-coding sequences, a decrease of pseudogenes from 139 to 53, and two inversions and one rearrangement corrected. We identified 30 virulence genes, none associated to the camel host, and the genes rpob2 and rbpA predicted to confer resistance to rifampin. In comparison to 129 genomes of the same species, strain Cp162 has four genes exclusively present, two of them code transposases and two truncated proteins, and the three exclusively absent genes lysG, NUDIX domain protein, and Hypothetical protein. All 130 genomes had the rifampin resistance genes rpob2 and rbpA. Our results found no unique gene that could be associated with tropism to camel host, and further studies should include more genomes and genome-wide association studies testing for genes and SNPs.

Funder

The Federal University of Minas Gerais, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Federal University of Pará

Publisher

PeerJ

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