Distinction of experimental wounds microcirculation under local immunotherapy

Abstract

Introduction. Changes in local microcirculation are essential in the treatment of infectious surgical diseases. Aim of the study to evaluate the features of microcirculation while comparing local treatment with aqueous dispersions of oxide nanostructures of metals, myelopide and chlorhexidine bigluconate in experimental conditions on animals.Materials and methods. Experimental modeling was performed on white Wistar rats of both sexes. 6 groups were formed: 1 – withdrawn from the experiment on the 1st day; 2 – control group without treatment; 3 – treatment with an aqueous dispersion of metal oxide nanostructures (group 1); 4 – treatment with a solution of 300 µg of myelopide per 1 ml (group 2); 5 – treatment with a solution of 600 μg of myelopide per 1 ml (group 3); 6. treatment with an aqueous solution of chlorhexidine bigluconate 0.05 % (group 4). The study was carried out on the 1st and 7th days of treatment, determining the number of capillaries per 0.1 mm2 of biopsy specimens. The data were processed with the determination of the mean values (M±m), 1st and 3rd quartiles [Q1-Q3], standard deviation (σ), correlation coefficient (CV, %), coefficient of Student’s.Results. On the first day, the animals had an average of 2.5±0.9 capillaries per 0.1 mm2 of wound area. On the 7th day, the lowest vascularization was in the control group 2.7±0.9 per 0.1 mm2 . The largest number of vessels was determined in the 1st observation group using aqueous dispersions of metal oxide nanostructures. The correlation coefficients were 40–56 %. On the 7th day, the values of neoangiogenesis during local immunocorrection using an aqueous solution of myelopide were similar.Conclusions. The least vascularization was noted in the control group (2.7±0.9). The greatest changes were associated with the use of aqueous dispersions of metal oxide nanostructures (3.8±1.4). The differences in the healing dynamic were associated with neoangiogenesis and the multifactorial nature of local immunocorrection.