Native mushrooms are valuable non-timber forest products used worldwide, although they are under-exploited in Africa. This study focused on evaluating ethnomycological practices in Nigeria. The study was based on structured questionnaires in three of Nigeria's six geopolitical regions. Findings revealed that mycophagy is not a gender-based activity (<i>P</i> ≤ 0.05) in all the sites assessed. There were no significant differences (<i>P</i> ≤ 0.05) between the sites evaluated, but the responses were quite different. Most interviewees (75.6%) ate mushrooms. Mushrooms were predominantly associated with delicious taste, exotic aroma, and flavor (37.0%), a meat substitute (37.0%), medicinal resources (20.3%). Among the principal medicinal mushrooms are <i>Lentinus squarrosulus, Pleurotus tuber-regium, Ganoderma lucidum, Auricularia auricula-judae</i>, and <i>Agaricus campestris</i>. Among the most important edible mushrooms are <i>L. squarrosulus and Termitomyces titanicus, Russula meleagris, A. campestris, and A. auricula-judae</i>. The main edible and medicinal dual-use mushrooms are <i>L. squarrosulus, P. tuber-regium, Lactifluus inversus, Polyporus officinalis</i>, and <i>T. titanicus</i>. Women and children (78.2%) are the major collectors/gatherers of mushrooms. The mushrooms are utilized to treat skin problems (21.5%), high blood pressure (12.3%), as blood tonic (9.2%), to treat fever and measles (13.8%), and diabetes (13.1%). Preservation practices include mainly direct sun-drying (48.7%) or washing and then sun-drying (27.8%). Mushrooms are consumed with 'fufu' (cereal-boiled cakes) (48.0%), and 'gari' (cassava-boiled cakes) (15.7%). Mushrooms are often made in soup (44.1%), cooked with meat (15.3%), or with vegetables (28.0%). Respondents were able to differentiate between toxic mushrooms and were familiar with some mushroom-related myths.