Correlation of some Immunological Markers with Systemic Lupus Erythematosus disease

Abstract

SLE (Systemic Lupus Erythematosus)  is, a heterogeneous disease, with ,diverse clinical manifestation disorder characterized by hyperactivity of  B and T cell, creation  of auto, antibodies, and, deposition, of antibody- containing immune complexes in blood vessels, during body. This study aims to investigation some immunological markers related to patients with SLE. It includes the study of the level, of Interleukin, (IL.17), antinuclear antibody (ANA), anti dsDNA, antiphospholipid and, complement, C3, C4, In addition to study the complete blood picture including hemoglobin, red blood cell monocytes, white blood cells, platelets and lymphocytes, they belong to factors that may interfere with the disease.  Current study included 110 patients with SLE, and 106 (96.36%) were female and 4 (3.64%) were male. Patients with an average age of 31.61 ± 8.60 years. This work also includes 70 blood specimens as control group. Our study illustrated that most infections were on Village 71.82 % compared with the City 28.18 , and patients without history disease  57.27 % while patients with history disease42.73%.    The results also showed that the concentration of IL.17 was significantly higher than p ≤ 0.05 in serum patients with lupus erythematosus (469 ± 2.25 g / ml)  comparison with healthy individuals (107.39 ± 0.41 pg / ml).  ELISA was used to measure some types of autoantibodies, and showed an increase in ANA and dsDNA Ab levels in SLE patients compared with healthy individuals. The results also prone that the antiphospholipid is significantly increase (P ≤ 0.05) in patients with SLE compared to controls.Evaluating serum complement  level C3, C4 in the total study, our results observed  significant decrease (p ≤ 0.05) in serum of patients with SLE (0.62 ± 0.01,0.13 ± 0.01g/l) respectively, compared with controls ,(1.74 ± 0.01,0.28 ± 0.01g/l) respectively.The results of the study also showed a significant decrease in hemoglobin, red blood cell monocytes, white blood cells, platelets and the number of lymphocytes for SLE patients compared with healthy individuals.The relationship between antibodies to ANA and IL-17, antibodies to dsDNA and C3, C4 and lymphocytes was identified, as well as the relationship between APA, C3 and C4 antibodies.    Inconclusion  ,peoples with SLE have a positive ANA, High  concentration of, ds DNA Ab and anti-phospholipid which plays an important role in pathogenesis and complement C3 and C4 in patients with SLE is decreased. An increased inflammatory concentration of interleukin (IL.17) can play a critical role in the pathogenesis of SLE which leads to disease progression.