Electrochemical Evaluation of Free Radical Scavenging Activity of Sesamol

Abstract

In this paper the evaluation of free radical scavenging activity of sesamol using a purine sensor was performed. The sensor was constructed via electro-immobilization of the guanine (Gua) on the surface of glassy carbon electrode. Guanine damage was induced by the hydroxyl radicals generated during Fenton-type reaction. Sesamol (Ses) which is an efficient antioxidant, well−known for its health−promoting properties was used in the present evaluation as a free radical scavenger. The interaction, carried out between the immobilized guanine and the free radicals, in the presence and absence of Ses was evaluated through changes in the Gua anodic peak. Under optimized conditions, the proposed sensor showed linear response in the sesamol concentration range from 0.1 to 0.6 mg l−1, the LOD value was equal to 0.02 mg l−1. The results clearly demonstrated that Ses has ability to scavenge the hydroxyl radical and protect the immobilized guanine.